ABSCN-5 Includes: SRV, SIV, STLV, Herpes B Surrogate Marker, Measles
ABSCN-8 Includes: SRV, SIV, STLV, Herpes B Surrogate Marker, Measles, RhCMV, SFV, RRV
ABSCN-Custom Includes: ABSCN-8 and addtional agents by request (SV40, LCV, custom).
The Antibody Screen panels utilize Multiplex Microbead ImmunoAssays to detect the host’s humoral immune response to various infectious agents. The panels simultaneously detect virus-specific antibodies in serum, plasma or other body fluids. The antibody screen assays are run on a Luminex platform using panels of microbeads coated with purified antigens. Each antigen bead has uniquely identifiable spectral signature.
Microbeads are incubated with diluted serum samples so that any specific antibodies present will bind to the matching antigen coated beads. The beads are washed, and reacted with phycoerythrin-conjugated detection reagents. After additional washing, independent gated events for each bead are counted and analyzed in a Luminex cytometer.
The median fluorescence intensity (MFI) of antigen-coated bead sets is compared to control coated beads in each serum. These assays were designed as screening assays with optimal sensitivity and require confirmatory testing by more specific methods.
The minimum sample requirement is 1 ml of serum or plasma sent frozen.